Journal: Advanced Materials (Deerfield Beach, Fla.)
Article Title: Ultrasound‐Responsive Dual‐Prodrug Nanoassembly for “Fenestrae‐Restoration Strategy” in Liver Fibrosis Therapy
doi: 10.1002/adma.202518832
Figure Lengend Snippet: Anti‐fibrotic effects and restoration of liver sinusoidal fenestrae by various formulations in CCl 4 ‐induced fibrotic mice. (a) Schematic illustration of liver fibrosis induction by CCl 4 in ICR mice and subsequent therapeutic interventions, followed by experiment termination. (b) Representative ultrasound images of the liver and kidneys. (c) Hepatorenal grayscale ratios across experimental groups. (d) Gross pathological images of livers after treatment. (e) Representative SEM images of sinusoids in livers after experiment termination. Scale bar, 1 µm. (f) Semiquantitative analysis of liver sinusoidal porosity. (g) Serum ALT and AST levels. (h) Hepatic hydroxyproline and (i) cGMP levels. (j) Sirius Red staining of collagen and corresponding quantitative analysis. Scale bar, 200 µm. (k) Masson's Trichrome staining of liver sections and corresponding quantitative analysis. Scale bar, 200 µm. (l) Immunohistochemical staining of α‐SMA and corresponding quantitative analysis. Data are expressed as mean ± SD ( n = 3 biologically independent samples). Groups: G1: Healthy control, G2: CCl 4 , G3: PMet, G4: PMS, G5: US, G6: PMS+US, G7: Met. ns: no significance, * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.
Article Snippet: SEM imaging was performed using JEOL JSM‐IT700HR (JEOL, Tokyo, Japan), and liver sinusoidal porosity was quantitatively analyzed using Image J.
Techniques: Staining, Immunohistochemical staining, Control
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